No.
At present, almost all operating systems use at least some Assembly. High-level interpreted or JIT-compiled languages have been used to write operating systems, but they inevitably require substantial portions to be implemented using Assembly or C.
There are not many programming languages that can be used to implement operating systems on their own. Ada, C, C++, Pascal, PL/1 are used for such purpose, but even they require some Assembly, or the operating system is highly specialised and limited in use (ie. for avionics, robots, etc., not general-purpose operating systems like Linux or Windows).
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