Choose this setting carefully! The memory you give to the VM will not be available to your host OS while the VM is running, so do not specify more than you can spare. For example, if your host machine has 1 GB of RAM and you enter 512 MB as the amount of RAM for a particular virtual machine, while that VM is running, you will only have 512 MB left for all the other software on your host. If you run two VMs at the same time, even more memory will be allocated for the second VM (which may not even be able to start if that memory is not available). On the other hand, you should specify as much as your guest OS (and your applications) will require to run properly.